Oops, we did it again.
This time with CNR: Nino Mazzaglia, Giuseppe Pappalardo and may others and of course our dear Milo Malanga.
Together we explored the role, chemistry and biology of defensins, of course with the help of cyclodextrins, particularly succinylated-BCD polymer.
Defensins are a large and ancient family of host–defence peptides present across vertebrates, invertebrates, and plants, where they play a crucial role in innate immunity.
Whatever you want, apparently 🙂
Our long-term collaboration with Salvo Sortino‘s team and Szabolcs Béni just yielded another great paper with Milo Malanga.
Here, we made a photoresponsive β-cyclodextrin branched polymer, where excitation of the Poly-βCD1 with visible blue light results in the generation of nitric oxide.
The negligible cytotoxic action of DOX, used well below the therapeutic doses, alone or in combination with the polymer in the dark, is enhanced in both cell lines under light irradiation exclusively when the drug is combined with Poly-βCD1 as a result of the combined action of NO.
Today’s cyclodextrin is a fascinating example of how to use the scavenging capabilities of cyclodextrins to develop tools against natural toxins by Alex Fragoso’s team.
Tetrodotoxins (TTXs) are potent marine neurotoxins involved in humans’ poisoning cases after consuming some marine organisms. In this work, five insoluble cyclodextrin polymers with different chemical structures have been investigated as novel clean-up materials for oyster extracts containing TTXs.
The best recoveries have been achieved with βCDP-14-DS2 (∼100 %), which allowed exposing cells to 27-fold higher oyster matrix concentrations and decreased the LOQ down to 46 µg equiv. TTX/kg.
Then, the applicability of this strategy has been demonstrated with oyster extracts from The Netherlands and comparing the results with immunoassay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). This clean-up strategy combined with CBA could be implemented for TTXs detection in monitoring programs to ensure seafood safety.
β-cyclodextrin polymer as tetrodotoxins scavenger in oyster extracts – ScienceDirect
Fascinating patent by Bernard Martel, Nicolas Blanchemain and colleagues presenting hydrogels on anionic cyclodextrin polymers and chitosan for use in the treatment of articular disorders. The hydrogel combines an analgesic action with a visco-supplementation (a lubricating effect).
WO2024023350 HYDROGEL COMPOSITION FOR USE IN THE TREATMENT OF ARTICULAR DISORDERS (wipo.int)
As our interest to use cyclodextrin in genetherapy as superior drugdelivery tools continue, we welcome papers like this, where cationic CD polymers are used for nucleotide formulation.
Leonard Wiebe and coworkers from University of Alberta development a carrier designed to effect selective transmembrane internalization of nucleotides via the asialoglycoprotein receptor.
Apparently, cyclodextrin-based oligonucleotide delivery is becoming a hot research topic. This recent study from University of Nova Gorica – Tina Škorjanc, PhD, Damjan Makuc, Nora Kulak, and Valant Matjaz presents a cyclodextrin porous polymer to form nanometer-sized particles and used as a delivery vehicle for metal-free and Cu(II)-metalated anthraquinone-based DNA intercalators.
Cyclodextrin polymers have increasing importance, as they are evaluated more and more frequenty in drug delivery solutions and non-pharma applications. This important paper deals with critical aspects of their behavior, such as interaction with human serum albumin, red blood cells bacteria.
Cyclodextrin derivatives and their polymers did not affect the secondary structure content of human serum albumin, which might mean a mild effect on the structural and functional properties of the main blood plasma protein.
Using the hemolysis test, it was found that polymers interact with red blood cells and can be assigned to non-hemolytic and slightly hemolytic groups as biocompatible materials, which are safe for in vivo use.
The cyclodextrins and their polymers did not extract proteins from bacterial cell walls and did not demonstrate any antibacterial activity against Gram-positive and Gram-negative strains.
today’s cyclodextrin:
we have already talked about both rotaxanes and CD-supported 3D printing Now Dartmouth College, an invention form Chenfeng Ke; Qianming Lin; Miao Tang and Li Longyu presents the combination of the two.
Rotaxanes have numerous applications, including applications in three-dimensional printing. However, current rotaxanes have numerous limitations in terms of fabrication and properties optimal for 3D printing. For instance, the synthesis of cyclodextrin-based three-dimensional (3D)—printable polypseudorotaxanes, which are formed by threading α-cyclodextrins (α-CDs) onto linear polyethylene glycol (PEG), requires the use of high molecular weight PEG with a minimal molecular weight of 10 k Pa.
Rotaxanes presented in this patent overcome such handicaps and offer a feasible solution.
See the full patent here on Espacenet
today’s cyclodextrin:
In this invention, BioNTech SE provides a method of producing a composition comprising RNA lipoplex particles. The main focus of the description is antitumoral therapy.
In the formulation itself, the aqueous colloidal suspension of liposomes is combined with an aqueous solution comprising RNA, thereby producing the composition comprising RNA lipoplex particles. The formulation requires frozen storage conditions; hence the use of cyroprotectant is necessary. The invention proposes mono- di- and oligosaccharides to protect such compositions, including various cyclodextrins and cyclodextrin polymers.
The use of cyclodextrins as cryoprotectants and stabilizers in biopharmaceutical formulations is an emerging technology.
Dr. Hossam Hefesha, Heinrich Haas, Ferdia Bates, Christian Hotz
Katalin Karikó
See the full patent Patentscope
The biomedical, biotech, and food industries all use polysaccharides as vital biomolecules with a variety of applications. Polysaccharides have great potential as biomaterials, and polysaccharide-based nanogels have attracted a lot of attention as carriers for different bioactive agents due to their unique versatile groups, specifically for site-directed or controlled delivery of the payload and, particularly because of their physicochemical properties, biodegradability, and biocompatibility. The 3D polymer network of NG produced by chemical crosslinking or physical self-assembly may be used to incorporate hydrophilic or hydrophobic molecules, including tiny drugs and proteins, genetic material patterns, and even ultrasmall nanoparticles. In this review, the chemistry of specific common polysaccharides and the various methods used to fabricate polysaccharide-based NG is discussed, with an emphasis on their use NG as drug delivery systems.
FOUAD DAMIRI, Dr.Satish Rojekar, Yahya Bachra, Rajender Varma, Dr. Swetha Andra, Satheesh kumar Balu, Dr. Chandrakantsing Pardeshi, Vijaysing Pardeshi, Hitendra Patel, Ana Cláudia Paiva-Santos, Mohammed Berrada, Mónica Cristina García
