Polyvinyl alcohol, N-acetylcysteine, and methyl-β-cyclodextrin exhibit albumin functions in natural killer cell culture

Today's cyclodextrin,

Today’s cyclodextrin is about replacing albumin with a magic combo of Polyvinyl alcohol, N-acetylcysteine, and methyl-β-cyclodextrin.
Albumin is a crucial component of serum-free media, playing a significant role in ex vivo cell culture as a lipid carrier and antioxidant.
Polyvinyl alcohol (PVA), N-acetylcysteine (NAC), and methyl-β-cyclodextrin (M-β-CD) were tested as an albumin substitute combination (PNM) and optimized their concentrations by using response surface methodology.
Verification of functions of PNM showed that intracellular fatty acid levels, cholesterol consumption rates, and the pSTAT5 level in the PNM group were significantly higher than those in the 0.75 g/L BSA group.

Reactive oxygen species levels remained controlled, and mitochondrial membrane potential was similar. These findings suggested that the PNM can effectively replace the functions of BSA as a fatty acid carrier, antioxidant, and, to some extent, a cholesterol carrier. This study provides insights for developing chemically defined media to prepare clinical-grade NK cells efficiently.

Polyvinyl alcohol, N-acetylcysteine, and methyl-β-cyclodextrin exhibit albumin functions in natural killer cell culture – ScienceDirect

“Biotechnologists’ Days Expo and Conference in Budapest

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We are going to participate in the “Biotechnologists’ Days Expo and Conference,” where university students can meet several companies in the biotech field in Hungary.
Tamas Kiss, will represent CarboHyde with Balázs Attila Kondoros and Tamás Vargadi.

Balázs will give a presentation, and we will also bring a poster to introduce our company and our biotech topics.

Hope to see many young talents there!

“Wash-free” synthesis of cyclodextrin metal–organic frameworks†

Egyéb kategória

Have you ever made a CD-MOF?

These guys make it seem pretty easy!

They propose a simple and fast synthetic strategy for preparing highly crystalline γ-cyclodextrin-based metal–organic frameworks (solid yield 100%). This is the first method that allows metal–organic frameworks with high surface areas to be synthesised without a washing step.

“Wash-free” synthesis of cyclodextrin metal–organic frameworks – RSC Mechanochemistry (RSC Publishing)

Theratechnologies Receives FDA Approval for EGRIFTA WR™

Today's cyclodextrin, drug delivery

Today’s yclodextrin:
I have often been asked about the CD-based products delivered subcutaneously by many partners.

I always had to refer to research and clinical trials, yet there were no marketed products.Now, that era is over, here you can see the first s.c. delivered, HPBCD-supported drug (Tesamorelin F8 by Theratechnologies Inc.) approved by the FDA:
Theratechnologies Receives FDA Approval for EGRIFTA WR™ (Tesamorelin F8) to Treat Excess Visceral Abdominal Fat in Adults with HIV and Lipodystrophy | Theratechnologies Inc.

Tesamorelin for injection is the only medication approved in the U.S. for the reduction of excess abdominal fat in adults with HIV who have lipodystrophy.


Exploring the Efficacy of Methylated Gamma-Cyclodextrin (M-γ-CD) in the Removal of Heavy Metals in Soil Systems

Today's cyclodextrin, ,

Today’s cyclodextrin is about the Removal of Heavy Metals in Soil Systems

This review provides a comprehensive overview of environmental remediation strategies using cyclodextrin and examines the effectiveness of methylated gamma-cyclodextrin (M-γ-CD) for the elimination of toxic elements from impure soil matrices.

M-γ-CD has emerged as a potent agent for treating soil impurities with noxious metals forming inclusion complexes with heavy-metal ions, thereby cumulating the aqueous solubility and efficiency of pollutants in environmental applications and improving soil bioremediation.

Dr Nontobeko Gloria Maphuhla and Dr OpeOluwa Oyedeji

Exploring the Efficacy of Methylated Gamma-Cyclodextrin (M-γ-CD) in the Removal of Heavy Metals in Soil Systems

A unique two-excipient heat stable tablet formulation of oral insulin: preliminary results

Today's cyclodextrin,

Today’s cyclodextrin is about stabilizing insulin.

Using freeze drying to form a heat-stable tablet in a single-step process, hydroxypropyl beta cyclodextrin (HP-β-CD) encapsulated lipophilic ion-pair complex of insulin using bile salt achieves demonstrated intestinal absorption and sustained glucose levels.

The tablets produced using this simple approach with only two excipients offer protection from enzymatic and stomach acid degradation and facilitate insulin uptake, without any need for specialized drug manufacturing or enteric coating.

The convenient presentation of insulin as a thermostable oral tablet presents a low-cost scalable manufacturing method that simplifies the logistics of storage, transport, and distribution in any setting, including areas where cold storage maybe limited or unavailable.


Read the full paper from Manshun Lai, Changcheng Zhu, Jaclyn Delarosa, and Manjari Lal PhD here: A unique two-excipient heat stable tablet formulation of oral insulin: preliminary results | bioRxiv