Differences in the Intracellular Localization of Methylated β-Cyclodextrins-Threaded Polyrotaxanes Lead to Different Cellular States

Today’s cyclodextrin is an essential paper on understanding differences in internalization and cellular location of various polyrotaxanes by Hokkaido UniversityJapan Science and Technology Agency (JST) and Tokyo Medical and Dental University

Activation of autophagy represents a potential therapeutic strategy for the treatment of diseases that are caused by the accumulation of defective proteins and the formation of abnormal organelles. Methylated β-cyclodextrins-threaded polyrotaxane (Me-PRX), a supramolecular structured polymer, induces autophagy by interacting with the endoplasmic reticulum. The same level of autophagy induction was achieved at one-twentieth the dosage for the mitochondria-targeted nanocarrier (Me-PRX) compared to the naked Me-PRX. The quantitative evaluation of the intracellular organelle localization of both naked Me-PRX and the MITO-Porter (Me-PRX) are reported here. Mitochondria, endoplasmic reticulum and lysosomes were selected as target organelles because they would be involved in autophagy induction. In addition, organelle injury and cell viability assays were performed. The results showed that the naked Me-PRX and the MITO-Porter (Me-PRX) were localized in different intracellular organelles, and organelle injury was different, depending on the route of administration, indicating that different organelles contribute to autophagy induction. These findings indicate that the organelle to which the autophagy-inducing molecules are delivered plays an important role in the level of induction of autophagy.

Biomolecules | Free Full-Text | Differences in the Intracellular Localization of Methylated β-Cyclodextrins-Threaded Polyrotaxanes Lead to Different Cellular States (mdpi.com)

Sustained Delivery of Cu(II)-Based DNA Intercalators by Nanometer-Sized Cyclodextrin-Based Porous Polymers

Apparently, cyclodextrin-based oligonucleotide delivery is becoming a hot research topic. This recent study from University of Nova Gorica – Tina Škorjanc, PhDDamjan MakucNora Kulak, and Valant Matjaz presents a cyclodextrin porous polymer to form nanometer-sized particles and used as a delivery vehicle for metal-free and Cu(II)-metalated anthraquinone-based DNA intercalators.

Sustained Delivery of Cu(II)-Based DNA Intercalators by Nanometer-Sized Cyclodextrin-Based Porous Polymers | ACS Applied Nano Materials

Functional group polarity-modulated formation of liquid crystals of amphiphilic cyclodextrins

Today’s cyclodextrin:
At CarboHyde, we also deal with amphiphilic CDs; this application from this Canadian collaboration caught my attention.
Pseudo-face-to-face symmetry in the native CDs represents a distinctive advantage to designing amphiphilic materials capable of self-assembly into liquid crystals. In this work, a new family of amphiphilic β-CD derivatives possessing 14 stearoyl chains (non-polar) and 7 functionalized tetraethylene glycols were synthesized using an improved design and more efficient chemistry, and the synthetic targets showed excellent ability to form stable hexagonal columnar mesophases. Studies of a lithium composite revealed fast local Li-ion exchange processes with very low activation energies, suggesting the benefit of using these materials as potential electrolytes for high ionic conductions. The results from this work can guide the design of future generations of CD-based LC materials for ion conduction.

University of Calgary – Austin Che, Simon Trudel-LachanceJayar EspejoChang-Chun Ling
University of Alberta – Diganta SarkarVladimir Michaelis
Simon Fraser University – Carson ZellmanVance Williams

See the full article here: Functional group polarity-modulated formation of liquid crystals of amphiphilic cyclodextrins