Site-specific modification of a glycoprotein through transglutaminase-mediated conjugation
Cool glyoco-invention got into the light from Zhaohui Sunny Zhou and Amissi Sadiki (Northeastern University), who study methods for making a site-specific modification of a glycoprotein that preserve the charge and glycoform of the glycoprotein. In short, one or more glycans are trimmed from the glycoprotein while keeping a core glycan attached to the glycoprotein. The removal of one or more glycans provides access to a targeted amino acid for a site-specific reaction. The attached core glycan can optionally be utilized for modulation of the glycoprotein, or one or more glycans or reagents can be attached to the core.