Synergistic effect of cyclodextrins and electrolytes at high concentrations on protein aggregation inhibition
Today’s cyclodextrin is about preventing protein aggregation.
I have posted a lot about how CDs can interact with proteins, CarboHyde even shared an educative deck on this earlier: CDs_in_protein_formulations.pdf
The research below from Kobe University and synplogen adds a new feature to this knowledge via exploring the Synergistic effect of cyclodextrins and electrolytes at high concentrations on protein aggregation inhibition:
Synergistic effect of cyclodextrins and electrolytes at high concentrations on protein aggregation inhibition – Journal of Pharmaceutical Sciences
Research highlights:
•High cyclodextrin (CD) and electrolyte concentrations synergistically stabilize biopharmaceutical proteins.
•HP-β-CD and Arg-HCl significantly increase the onset temperature of protein aggregation of immunoglobulin G, insulin, and adeno-associated virus vectors.
•This synergistic effect was not observed when sucrose was used instead of HP-β-CD.
•HP-β-CD and Arg-HCl combination effectively inhibits the formation of soluble and insoluble IgG aggregates during long-term storage.
