Cyclodextrin glucanotransferase: fundamentals and biotechnological implications

Today's cyclodextrin,

Today’s cyclodextrin:
is about the birth of these compounds. I collected two great sources; first, a review that presents a comprehensive view of CGTase produced by Bacillus spp. and other bacterial genera like Klebsiella, Paenibacillus, and Microbacterium. It also gives insight of the properties and recent biotechnological applications of cyclodextrins.

Cyclodextrin glucanotransferase: fundamentals and biotechnological implications | SpringerLink


However, the bioproduction of CDs still faces challenges in terms of optimization and process complexity. The 2nd study proposes a novel CD bioproduction system in a batch mode to increase yield and reduce costs. Scale-up to the bioreactor displayed improved growth kinetics for both bacteria and higher protein production and CGTase activity for Paenibacillus macerans.


Future Pharmacology | Free Full-Text | Design of a Cyclodextrin Bioproduction Process Using Bacillus pseudofirmus and Paenibacillus macerans (mdpi.com)


Use of cyclodextrin to enhance solubility of substrates and increase enzymatic glycosylation reaction efficiency

carbohydrates,

We are particularly fond of biotech applications of #cyclodextrin, and the invention of DoubleRainbow Biosciences gives a simple solution for exploiting their potential in this field and helps understand how CDs could be used to improve enzymatic glycosylation efficiency.
Yasmin Chau, Jacob Wirth, DING, Sheng, Jing-Ke Weng

See the full patent on Patentscope: Use of cyclodextrin to enhance solubility of substrates and increase enzymatic glycosylation reaction efficiency

In Utero Enzyme-Replacement Therapy for Infantile-Onset Pompe’s Disease

carbohydrates, ,

Amazing therapeutic success led by UCSF Pediatrics joined by Duke University, Ottawa University, and Washington University via in-utero enzyme-replacement therapy for infantile-onset Pompe’s disease: Patients with early-onset lysosomal storage diseases are ideal candidates for prenatal therapy because organ damage starts in utero. After receiving in-utero ERT and standard postnatal therapy, the current patient had normal cardiac and age-appropriate motor function postnatally, was meeting developmental milestones, had normal biomarker levels, and was feeding and growing well at 13 months of age.

See the full article here

Design, Synthesis, and Characterization of Stapled Oligosaccharides

carbohydrates, , ,

From peptides to sugars: Design, Synthesis, and Characterization of Stapled Oligosaccharides. Oligosaccharide stapling increases enzymatic stability and cell penetration, opening new opportunities for using glycans in medicinal chemistry. – Max Planck Institute Manuel Garcia Ricardo, Emelie Ellen Reuber, Ling Yao, José Angel Danglad-Flores, Martina Delbianco, Peter Seeberger
Wonderful chemistry and insightful new possibilities.

See the full article here