Whole-body cellular mapping in mouse using standard IgG antibodies

Fascinating new method, wildDISCO, uses 2,6-dimethyl-β-cyclodextrin to overcome the limitations of conventional whole-body imaging methods.

This technology enables the uniform staining of whole bodies with conventional IgG antibodies and reveals cellular and structural maps across the entire mouse body. This has allowed us to map diverse biological systems, including peripheral nerve atlases and the complete lymphatic system, and make novel biological findings. We studied tertiary lymphoid structures (TLS) in the whole mouse body along with cancer metastasis, providing insights into their therapeutic potentials.

The wildDISCO method facilitates the visualization of endogenous proteins in a whole body without a transgenic reporter. Its compatibility with over 30 commercial antibodies marks a breakthrough in biomedical applications and research at the whole mouse level.
Rami Al-MaskariIzabela HorvathMarie PiraudJohannes C. PaetzoldJennifer ModamioFarida Hellal & Ali Ertürk et al.

See the full article on nature.com!

Developed an Indocyanine / β-cyclodextrin conjugate for ureter visualization

Today’s cyclodextrin:
It is in the field of imaging. Mie University and Nagoya University has jointly developed an Indocyanine / β-cyclodextrin conjugate suitable for ureter visualization in patients undergoing laparoscopic/minimally invasive colorectal surgery. 
Astellas Pharma has acquired exclusive development and marketing rights worldwide (the designation of the compound is ASP5354). A recent clinical trial investigated the safety, tolerability and the pharmacokinetics of ASP5354 in participants undergoing laparoscopic/minimally invasive surgery.
ASP5354 fluoresces upon excitation with a particular wavelength of near-infrared light. When administered intravenously, ASP5354 is primarily and rapidly excreted by the kidneys and provides the surgeon with visualization of the ureter(s) during surgery through the use of a near-infrared fluorescence (NIR-F) medical device.

Please follow these links to read the corresponding article!

A Study for Ureter Visualization, Using ASP5354 in Subjects Undergoing Laparoscopic/Minimally Invasive Colorectal Surgery

Ready vascular permeability of a near‑infrared fluorescent agent ASP5354 for intraoperative ureteral identification enables imaging of carcinoma tissues.

Insights into recent preclinical studies on labeled cyclodextrin-based imaging probes: Towards a novel oncological era

today’s cyclodextrin:
is a beautiful review on labeled CD-based imaging probes from University of Debrecen (Zita Képes, István Hajdu, Ferenc Fenyvesi, and György Trencsényi).
As malignancies remain one of the major health concerns worldwide, increasing focus has been centered on the application of cyclodextrins (CDs) in cancer imaging and therapy due to their outstanding inclusion-forming capability. Studies applying fluorescently- CDs, or CD-based MRI contrast agents revealed much about pharmacokinetics and diagnostic applications; however, derivatives labeled with positron emitters seem superior molecular probes in the investigation of the route of CDs in the biological niche. Exploiting the firm signaling interaction between cancer-related cyclooxygenase-2, prostaglandin E2 (PGE2), and RAS oncoprotein, radioconjugated, PGE2-affine CDs project the establishment of novel imaging probes and therapeutic agents. 

See the full article here: Insights into recent preclinical studies on labelled cyclodextrin-based imaging probes: Towards a novel oncological era