Today’s cyclodextrin is about fatty acid supplementation to cells.
In this study, Bradley Priem, Maciek Antoniewicz and colleagues from University of Michigan developed a robust method for supplementing fatty acids in CHO cultures using methyl-β-cyclodextrin (MBCD), and showed that the degree of saturation in CHO cell mass can be precisely tuned with tailored feeding strategies.
Using GC-MS and 13C tracing, we uncovered how supplemented fatty acids undergo elongation, desaturation, and β-oxidation—leading to striking changes in cell composition.
Fascinating work that paves the way for new feed media formulations containing fatty acids to optimize CHO cell physiology in industrial cell cultures.
